Composite

Part:BBa_K4179019:Design

Designed by: Amit Nelkin   Group: iGEM22_Technion-Israel   (2022-10-06)


XimD + XimE under T7 promoters + LacI + Lac operator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1772
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1772
    Illegal NheI site found at 1502
    Illegal NheI site found at 1995
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1772
    Illegal BglII site found at 3282
    Illegal BamHI site found at 1457
    Illegal XhoI site found at 1189
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1772
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1772
    Illegal NgoMIV site found at 178
    Illegal NgoMIV site found at 1237
    Illegal AgeI site found at 1751
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences of XimD&XimE were taken from a previous workthe work of Bu et al.



Source

XimD & XimE - Streptomyces xiamenensis strain 318 LacI - E. coli

References